Genome-Scale Identification Method Applied to Find Cryptic Aminoglycoside Resistance Genes in Pseudomonas aeruginosa

Genome-Scale Identification Method Applied to Find Cryptic Aminoglycoside Resistance Genes in Pseudomonas aeruginosa

BACKGROUND The ability of bacteria to rapidly evolve resistance to antibiotics is a critical public health problem. Resistance leads to increased disease severity and death rates, as well as imposes pressure towards the discovery and development of new antibiotic therapies. Improving understanding of the evolution and genetic basis of resistance is a fundamental goal in the field of microbiolog...

Aminoglycoside resistance in Pseudomonas aeruginosa.

Lability of Resistance to Aminoglycoside Antibiotics in Pseudomonas Aeruginosa

The determinants for resistance to aminoglycoside antibiotics in some strains of Pseudomonas aeruginosa were irreversively lost during storage or by treatment with ethidium bromide. This fact has indicated the presence of plasmids governing resistance to aminoglycosides in the pseudomonads as well as R factor in gram-negative enteric bacteria and plasmid in staphylococci. Many papers have descr...

In vitro characterization of aminoglycoside adaptive resistance in Pseudomonas aeruginosa.

Aminoglycoside adaptive resistance was characterized in one reference strain and four clinical isolates of Pseudomonas aeruginosa. Adaptive resistance was initiated with a 2-h gentamicin or tobramycin exposure at the MIC. Each P. aeruginosa strain demonstrated an adaptive-resistance period of between 8 and 12 h when tested with both aminoglycosides. Aminoglycoside adaptive resistance was shown ...

Genome-Scale Identification of Resistance Functions in Pseudomonas aeruginosa Using Tn-seq

We describe a deep-sequencing procedure for tracking large numbers of transposon mutants of Pseudomonas aeruginosa. The procedure employs a new Tn-seq methodology based on the generation and amplification of single-strand circles carrying transposon junction sequences (the Tn-seq circle method), a method which can be used with virtually any transposon. The procedure reliably identified more tha...